Kanamycin vs. Streptomycin (establishing effectiveness):  
We spread the bacteria C600 on 4 plates, and bacteria from a rollie pollie on 4 others.  Then we added 140 mcl of either Kanamycin, Streptomycin and a mystery antibiotic on six out of eight plates.  These results showed that all of the antibiotic Colloidal Silver had an effect on the C600, but were not successful on the bug juice.

Colloidal Silver, Kanamycin and test of potency:  25 microliters of C600 were put onto the plates, and then 5 of them had Colloidal Silver or Kanamycin dropped in the center of them. The others were mixed with the following: 5mcl Colloidal Silver, 50mcl Colloidal Silver, 100mcl Colloidal Silver, 5mcl K, or 50mcl K.  They were incubated and then the results were observed the next day.  The more Colloidal Silver that we put on the plate, the less bacteria grew. The Kanamycin produced similar results.  It too eliminated the bacteria, but it seemed to be less powerful at 5mcl than the Colloidal Silver. For the 50mcl, the Kanamycin was more powerful and wiped out more colonies than the Colloidal Silver.

Colloidal Silver vs. Water:  There weren’t any circular areas that could be measured by a ruler.  We thought that cutting a small circle in the middle of the agar plate, creating a little moat, would create an equal opportunity for the antibiotic to spread. We spread C600 over the plates, cut the moat and then put 10mcl of Colloidal Silver in the middle of one plate and distilled water on the other. The results revealed a figure that we could measure, and were able to calculate the area of the killing circle.

Fruit Flies:  We put a fly in an ependorf tube along with 50mcl of distilled water and homogenized it with a pestle.  50mcl of the bug mixture was then pipetted onto and agar plate and incubated at 37º.  Then we made a control plate by pipetting 50mcl of water onto a plate.  The water was first pipetted into an epindorf tube in order to pipette it into the agar plate.  We discovered that there we a lot of bacteria in just the water that we used and later discovered that the ependorf tubes were also contaminated.  The same type of bacteria was growing on each plate.

Fly Food: To test the Colloidal Silver and its toxicity we mixed water and fly food in one tube, and Colloidal Silver and fly food in another.  They were allowed to eat and when we observed them the following week, no new bugs had died.  So Colloidal Silver was not toxic.  Then we homogenized the bugs in sterile water and pippetted them onto plates.  5 flies were taken from each tube.  The flies that had been eating the Colloidal Silver had a significantly smaller number of bacteria colonies growing on them.

Dilution:  We diluted the Streptomycin and then compared it to Colloidal Silver.  Duplicates were done for each test.  They had either: just water, Colloidal Silver (full strength), Streptomycin (full strength), 10mcl water + 10mcl silver, or 20mcl water + 10mcl silver.  C600 was spread on every plate and then “moats” were made so the results could be measured.  There was killing at all levels of concentrations.  They were almost equal in results proving that antibiotic Colloidal Silver can be diluted and still be powerful.